Crispr Genetic Editing Methods In Target Diseases

[LeeAndro]

Published 8/2022MP4 | Video: h264, 1280x720 | Audio: AAC, 44.1 KHzLanguage: English | Size: 1.04 GB | Duration: 2h 33m

CRISPR/Cas Systems, Applications and Project Designs

What you'll learn
You will learn genetic treatment methods in target diseases with CRISPR-Cas approaches.​

Design CRISPR Cas project
Design guide RNA, Decision of best Cas system
Base editing, P Editing, Transposase and Knockout systems
Requirements
Participating in the RadiChal competition
Description
The CRISPR revolution has begun and shows no signs of slowing down. This system, which is the key to prokaryotic adaptive immunity, has proven to be particularly under the influence of genome eeering. CRISPR/Cas provides flexibility as well as easily amplifying the signal and regulating the signal level, which is beyond the capabilities of previous systems. Prokaryotes have long exploited CRISPR/Cas as a powerful defense strategy against virus-derived invaders, and eventually, this system turns out to be useful only for research applications.Just when we thought that genome eeering could not get any further, the CRISPR (clustered regularly spaced short palindromic repeats)/Cas9 system emerged. It is an important component of the bacterial immune system, allowing bacteria to recognize and destroy phages. In genome eeering applications, guide RNA (gRNA) sequence homology targets the Cas9 endonuclease to a specific locus-where the double helix is broken. Like ZFNs and TALENs, CRISPR/Cas9 uses HDR, but using RNA to detee editing makes the system more sensitive and malleable, as well as less expensive and -consuming. For this reason, CRISPR/Cas9 has proven invaluable for high-throughput genome eeering.With a variety of robust and effective genome eeering methods at our fingertips, we have entered the Golden Age of genome eeering. Current studies have focused on developing these techniques in the hope that they will be clinically useful to provide desired target loci (or loci), high specificity, and efficacy. The most exciting thing we've found at Addgene is the democratization of genome eeering that has enabled and will continue to enable researchers around the world to use these tools. To learn all about CRISPR, you must complete the course.CRISPR/Cas Gene Editing Training Programi. What is CRISPR-Casii. What are CRISPR-Cas Systemsiii. CRISPR Genetic Therapy Applicationsiv. Planning Your CRISPR Projectv. Guide RNA Design & Benchlingvi. Confirmation of your Gene Editing

Overview

Section 1: Introduction: What is CRISPR/Cas

Lecture 1 Introduction: What is CRISPR/Cas

Section 2: Chapter 2: What are CRISPR/Cas Systems

Lecture 2 chapter 2

Lecture 3 chapter 2 continue

Section 3: chapter 3: CRISPR/Cas Genetic Therapy Applications

Lecture 4 chapter 3

Section 4: chapter 4: Designing a CRISPR/Cas Gene Editing Project

Lecture 5 chapter 4

Section 5: chapter 5: CRISPR/Cas Gene Editing Confirmation Methods

Lecture 6 chapter 5

Medicine, Molecular Biology and Genetics and Biology departments

HomePage:

Https://anonymz.com/https://www.udemy.com/course/crispr-genetic-editing-methods/

DOWNLOAD

https://1dl.net/dngm3e9fw6d1/6yXML6BE__CRISPR_gen.part1.rar.html
https://1dl.net/9n5dofbs5rv4/6yXML6BE__CRISPR_gen.part2.rar.html